Protein Structure Determination

This lecture covers the methods of protein structure determination using Nuclear Magnetic Resonance (NMR) and Cryo-Electron Microscopy (Cryo-EM). It explains how NMR spectroscopy is used to determine the structure of organic molecules, focusing on chemical shifts and isotope labeling. The process of obtaining a protein structure through NMR spectroscopy, including data collection and analysis, is detailed. The lecture also delves into the principles of NMR spectroscopy, such as the Nuclear Overhauser Effect (NOE) and how to convert experimental data into a protein structure. Additionally, it introduces Cryo-EM as a technique for high-resolution protein structure determination, discussing sample preparation, imaging, and data processing.