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This lecture covers the fundamental aspects of enzymology, including enzyme structure, kinetics, and inhibition. Topics include enzyme characteristics, protein structure hierarchy, Michaelis-Menten equation, environmental factors affecting enzymatic reactions, and enzyme specificity. The lecture also discusses the catalytic power of enzymes, reversible reactions, and the coupling of endergonic and exergonic reactions. Various types of enzyme inhibition, such as competitive, non-competitive, and uncompetitive, are explored. Additionally, the lecture delves into multi-substrate enzymatic reactions and enzyme specificity. Practical applications and future directions in enzymology are also highlighted.