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This lecture covers the study of early embryogenesis using blastocoelic cavity, trophoblast, blastomer, and cell junctions, as well as the differentiation of ES cells into various cell types like cardiac muscle, neurons, and skin cells. It also explores disease modeling using pluripotent stem cells, investigating neuronal function in schizophrenic patients, genome editing technologies, and the creation of disease-specific in vitro models. The lecture delves into the generation of 'minibrains' from human ES cells to model human diseases, in vitro human retina and intestine from stem cells, single-cell transcriptome analysis, and lineage tracing using CRISPR-Cas9 technology.