RNA-based Probes: Engineering GFP-mimics

This lecture delves into the engineering and application of RNA-based probes, focusing on GFP-mimics. Starting with a refresher on fluorescent proteins like GFP, it explores the chromophore maturation process, autocatalytic mechanisms, and key structural features. The lecture then discusses the evolution of Spinach as a GFP-mimic for RNA tracking, comparing it with GFP fusion proteins. It covers the structure and mechanism of RNA mimics of GFP, highlighting key papers and the effects of deprotonation on the GFP chromophore. The session concludes with discussions on SELEX technology, binding considerations, and the utility of Spinach in RNA imaging.