Protein Assembly Dynamics: AFM Applications

This lecture explores the applications of Atomic Force Microscopy (AFM) in studying protein assembly dynamics, focusing on the core-complexes of reaction centers and light harvesting complexes. It covers imaging native membranes, AFM raw data topographs, and the localization of specific protein subunits. The lecture delves into the high-resolution AFM topographs, the heterogeneity of LH2 complexes, and the adaptation of membranes to different light conditions. Additionally, it discusses force spectroscopy AFM for protein unfolding and cellular interactions, as well as the characterization of tissues using AFM. The lecture concludes with insights into high-speed AFM imaging of protein assembly dynamics and the imaging of live bacteria at the nanoscale.