Publications by Tristan Colomb

Versatile spectral modulation of a broadband source for digital holographic microscopy

Christophe Moser, Tristan Colomb, Gaël Nardin

We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a ‘pulse-shaping’ geometry, the spatial light modulator provides a versatile control over the bandwidth and w ...

Optical Society of America2016

Digital holographic reflectometry

Christian Depeursinge, Tristan Colomb, Jonas Kühn, Nicolas Pavillon, Ahmet Ata Akatay

Digital holographic microscopy (DHM) is an interferometric technique that allows real-time imaging of the entire complex optical wavefront (amplitude and phase) reflected by or transmitted through a sample. To our knowledge, only the quantitative phase is ...

2010

Extended depth-of-focus by digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Viesturs Simanis, Pierre Marquet, Jonas Kühn, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Christian Depeursinge, Tristan Colomb, Frédéric Montfort, Benjamin Rappaz, Pierre Marquet, Nicolas Pavillon, Jonas Kühn, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Christian Depeursinge, Tristan Colomb, Pierre Marquet, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Axial sub-nanometer accuracy in digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn

2008

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Christian Depeursinge, Tristan Colomb, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Pierre Marquet, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007