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Michael Stefan Daniel Liebling

Related publications (21)

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From Nano to Macro An overview of the IEEE Bio Image and Signal Processing Technical Committee

Michaël Unser, Dimitri Nestor Alice Van De Ville, Michael Stefan Daniel Liebling

The Bio Image and Signal Processing (BISP) Technical Committee (TC) of the IEEE Signal Processing Society (SPS) promotes activities within the broad technical field of biomedical image and signal processing. Areas of interest include medical and biological ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2023

Estimating Nonplanar Flow from 2D Motion-blurred Widefield Microscopy Images via Deep Learning

Michael Stefan Daniel Liebling, Adrian Shajkofci

Optical flow is a method aimed at predicting the movement velocity of any pixel in the image and is used in medicine and biology to estimate flow of particles in organs or organelles. However, a precise optical flow measurement requires images taken at hig ...

IEEE2021

Significance: Despite recent developments in microscopy, temporal aliasing can arise when imaging dynamic samples. Modern sampling frameworks, such as generalized sampling, mitigate aliasing but require measurement of temporally overlapping and potentially ...

SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS2020

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

DeepFocus: a Few-shot Microscope Slide Auto-Focus using a Sample Invariant CNN-based Sharpness Function

Michael Stefan Daniel Liebling, Adrian Shajkofci

Autofocus (AF) methods are extensively used in biomicroscopy, for example to acquire timelapses, where the imaged objects tend to drift out of focus. AF algorithms determine an optimal distance by which to move the sample back into the focal plane. Current ...

2020

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with high-resolution ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2020

Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

Reconstruction of image sequences from ungated and scanning-aberrated laser scanning microscopy images of the beating heart

Michael Stefan Daniel Liebling, Olivia Mariani

Fluorescence laser-scanning microscopy is a well-established imaging technique in biology, available in many imaging facilities to investigate structures within live animal embryos such as zebrafish. Laser scanning microscopes (LSM) are limited when used t ...

2019

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overl ...

SPIE2019

Multi-Spectral Widefield Microscopy of the Beating Heart through Post-Acquisition Synchronization and Unmixing

Michael Stefan Daniel Liebling, Christian Jaques

Multi-spectral imaging allows distinguishing biological structures. For cardiac microscopy, available devices are either too slow or require illumination intensities that are detrimental to the sample. We present a method for spectral super-resolution imag ...

IEEE2019