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Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-col ...
An instrument for high-speed laser perfusion imaging comprises a laser source, a detector, a signal-processing unit, data memory, and a screen to display the results. A section of the sample surface is illuminated with laser light; the reemitted light from ...
Laser Doppler Imaging (LDI) is a non-invasive optical technique used for assessing the blood flow in biological tissue. This technique allows visualizing 2D distribution of blood flow over an extended area of tissue, e.g. human skin. We have developed a ne ...
We present a combination of topography measurements based on digital fringe projection and blood flow imaging based on Laser Doppler Imaging (LDI). Both techniques are optical, non-contact and high-speed whole-field methods well suited for in-vivo measurem ...
We present a fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral is evaluated with the fast Fourier transform for calculat ...
The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample (12) containing luminescent molecul ...
We describe the design and performance of a new full-field high-speed laser Doppler imaging system developed for mapping and monitoring of blood flow in biological tissue. The total imaging time for 256x256 pixels region of interest is 1.2 seconds. An inte ...
The combination of wavelength multiplexing and spectral interferometry allows for the encoding of multidimensional information and its transmission over a mono-dimensional channel; for example, measurements of a surface's topography acquired through a mono ...
We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epi-illumination configurati ...
We designed a fluorescence correlation spectroscopy (FCS) system for measurements on surfaces. The system consists of an objective-type total internal reflection fluorescence (TIRF) microscopy setup, adapted to measure FCS. Here, the fluorescence exciting ...
