Publications by Natalie Muller

Role of retroviral restriction factors in the interferon- -mediated suppression of HIV-1 in vivo

Jacques Fellay, Amalio Telenti, Natalie Muller, Patrick Taffe

The antiviral potency of the cytokine IFN-α has been long appreciated but remains poorly understood. A number of studies have suggested that induction of the apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3 (APOBEC3) and bone marrow stromal ce ...

National Academy of Sciences2012

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, David Hacker, Madiha Derouazi, Martin Jordan, Natalie Muller, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Orbital shaker technology for the cultivation of mammalian cells in suspension

Florian Maria Wurm, David Hacker, Martin Jordan, Natalie Muller, Philippe Pierre Girard

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Serum-free large-scale transient transfection of CHO cells

Florian Maria Wurm, Madiha Derouazi, Natalie Muller, Martin Bertschinger, Philippe Pierre Girard

To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies ...

2004

Calfection: a novel gene transfer method for suspension cells

Florian Maria Wurm, Martin Jordan, Natalie Muller, Philippe Pierre Girard

We have developed a novel method called Calfection for gene delivery to and protein expression from suspension-cultivated mammalian cells. Plasmid DNA was simply diluted into a calcium chloride solution and then added to the cell culture for transfection. ...

2004

Solvatochromic Analysis of Partition Coefficients in the o-Nitrophenyl Octyl Ether (o-NPOE)/Water System

Hubert Girault, Pierre-Alain Carrupt, Natalie Muller

The objective of this study was to unravel the structural properties responsible for the partitioning of solutes in o‐nitrophenyl octyl ether (o‐NPOE)/H2O, a new solvent system for the determination of the partition coefficients of ions. A set of 88 compou ...

2003

NMR studies of an oligoproline-containing peptide analogue that binds specifically to the H-2Kd histocompatibility molecule

David Lyndon Emsley, Geoffrey Bodenhausen, Natalie Muller

T lymphocytes expressing variable cell surface antigen receptors recognize "processed" forms of antigen, presented on the surface of other cells by molecules of the major histocompatibility complex (MHC). Naturally processed antigenic peptides can be repla ...

1991

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Natalie Muller

Role of retroviral restriction factors in the interferon- -mediated suppression of HIV-1 in vivo

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Transient gene expression for rapid protein production

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Orbital shaker technology for the cultivation of mammalian cells in suspension

Serum-free large-scale transient transfection of CHO cells

Calfection: a novel gene transfer method for suspension cells

Solvatochromic Analysis of Partition Coefficients in the o-Nitrophenyl Octyl Ether (o-NPOE)/Water System

NMR studies of an oligoproline-containing peptide analogue that binds specifically to the H-2Kd histocompatibility molecule

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Chat with Graph Search

Solvatochromic Analysis of Partition Coefficients in the o-Nitrophenyl Octyl Ether (o-NPOE)/Water System

Role of retroviral restriction factors in the interferon- -mediated suppression of HIV-1 in vivo

Calfection: a novel gene transfer method for suspension cells

NMR studies of an oligoproline-containing peptide analogue that binds specifically to the H-2Kd histocompatibility molecule

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Transient gene expression for rapid protein production

Orbital shaker technology for the cultivation of mammalian cells in suspension

Serum-free large-scale transient transfection of CHO cells