Publications de Natalie Muller

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, David Hacker, Martin Jordan, Madiha Derouazi, Natalie Muller, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

Orbital shaker technology for the cultivation of mammalian cells in suspension

Florian Maria Wurm, David Hacker, Martin Jordan, Natalie Muller, Philippe Pierre Girard

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Transient gene expression for rapid protein production : studies & optimizations under serum-free conditions

Natalie Muller

Recombinant proteins are important for biomedical research and for the treatment of human disease. Therefore it is necessary to develop reproducible bioprocesses to rapidly produce proteins of adequate quality and quantity. Expression in mammalian cells is ...

EPFL2005

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Martin Jordan, Natalie Muller, Philippe Pierre Girard, Frédéric Grosjean, Jason Wright

Springer2002