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Niels Erik Lion

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Plate for matrix-assisted laser desorption ionization mass spectrometry, comprises electroconductive substrate covered with sintered nanoparticles, deposited as array of spots, for supporting ionization of sorbed sample molecules

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao, Michel Prudent, Jingjing Wan

NOVELTY - A plate comprises an electroconductive substrate (1) at least partially covered with sintered nanoparticles (2), deposited as an array of individual spots, for use as a sorbing phase for a sample, and for supporting ionization of sorbed sample mo ...

2009

Iontophoretic Fraction Collection for Coupling Capillary Zone Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Niels Erik Lion

An automated fraction collection interface has been developed for coupling capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). This fraction collection approach is based on electromigration and diff ...

2009

About the Electrospray Ionization Source in Mass Spectrometry: Electrochemistry and On-chip Reactions

Hubert Girault, Niels Erik Lion, Christophe Roussel, Manuel Alejandro Méndez Agudelo, Michel Prudent, Joël Stéphane Rossier, Bin Su

The present work shows that the electrochemical properties of electrospray ionization (ESI) can be used to add functions to the process. As example, we show how the choice of the electrode material can be used to study interactions between metal ions and b ...

2009

Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies

Hubert Girault, Niels Erik Lion, Loïc Dayon, Mélanie Abonnenc, Brice Perruche

An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid ...

2008

Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides

Hubert Girault, Niels Erik Lion, Michel Prudent, Joël Stéphane Rossier

Phosphopeptides tagging reactions by dinuclear zinc(II) complexes (1,3-bis[bis(2-pyridylmethyl)amino]-propan- 2-olato dizinc(II)3+, called tag) were performed with a dual-channel microsprayer in electrospray ionization mass spectrometry. The reaction is fi ...

2008

Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao

NOVELTY - The plate has electrically conductive substrate (1) that is covered with light sensitive matrix (2). The matrix comprises light absorber, charge carrier, probe molecule and photo-sensitizer (3) arranged to oxidize probe molecule by irradiation of ...

2008

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools

Niels Erik Lion, David Crettaz, Olivier Rubin

Background and Objectives Microparticles (MPs) are small phospholipid vesicles of less than 1 mu m, shed in blood flow by various cell types. These MPs are involved in several biological processes and diseases. MPs have also been detected in blood products ...

Wiley-Blackwell2008

Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell

Hubert Girault, Niels Erik Lion, Hoang-Trang Lam

In proteomic analysis of complex samples at the peptide level (termed shotgun proteomics), an effective prefractionation is crucial to decrease the complexity of the peptide mixture for further analysis. In this perspective, the high-resolving power of the ...

American Chemical Society2007

Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation

Hubert Girault, Niels Erik Lion

Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum a ...

2007

Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy

Hubert Girault, Niels Erik Lion

Scanning Electrochemical Microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained us ...

2007

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Iontophoretic Fraction Collection for Coupling Capillary Zone Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Niels Erik Lion

2009

Plate for matrix-assisted laser desorption ionization mass spectrometry, comprises electroconductive substrate covered with sintered nanoparticles, deposited as array of spots, for supporting ionization of sorbed sample molecules

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao, Michel Prudent, Jingjing Wan

2009

Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao

2008

Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy

Hubert Girault, Niels Erik Lion

2007