Niels Erik Lion

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Microfluidic front-end technologies for protein electrospray mass spectrometry

In the present work, a polymer microfabricated microsprayer is characterised and tested for the mass spectrometric analysis of biomolecules, in particular in the context of protein mass spectrometry and proteomics. As the core of this work deals with analy ...

EPFL2006

Multitrack electrospray chips

Hubert Girault, Niels Erik Lion, Loïc Dayon, Michel Prudent, Mélanie Abonnenc

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass ...

Wiley-Blackwell2006

Chip electrospray mass spectrometry for carbohydrate analysis

Hubert Girault, Niels Erik Lion

Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, a ...

2005

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Hubert Girault, Niels Erik Lion, Joël Stéphane Rossier

A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucid ...

Royal Society of Chemistry2005

Editorial: Microfluidics in system biology

Hubert Girault, Niels Erik Lion, Joël Stéphane Rossier

2005

Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes

Hubert Girault, Niels Erik Lion

Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. Th ...

2005

Salt Removal During Off-GelTM Electrophoresis of Protein Samples

Hubert Girault, Niels Erik Lion, Christophe Roussel

The Off-Gel™ technology was recently described for protein fractionation in a solution placed on top of an immobilized pH gradient gel. In addition, this process was found to remove salts from the biological samples to analyze. This desalting effect is stu ...

2005

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Niels Erik Lion, Christophe Roussel, Loïc Dayon, Michel Prudent

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

2005

Thin-Chip Microspray System for High-Performance Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry of Biopolymers

Hubert Girault, Niels Erik Lion, Joël Stéphane Rossier

Wiley-VCH Verlag GmbH2003

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On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Niels Erik Lion, Christophe Roussel, Loïc Dayon, Michel Prudent

2005

Thin-Chip Microspray System for High-Performance Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry of Biopolymers

Hubert Girault, Niels Erik Lion, Joël Stéphane Rossier

Wiley-VCH Verlag GmbH2003

Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes

Hubert Girault, Niels Erik Lion

2005