Publications by Paolo Silacci | EPFL Graph Search

Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells

Martinus Gijs, Paolo Silacci, Hüseyin Cumhur Tekin, Josias Basil Wacker, Christine Ruffert, Guy Vergères

The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images

Giovanni De Micheli, Sandro Carrara, Julien Michel Ghaye, Danilo Demarchi, Paolo Silacci, Chiara Succa, Guy Vergères

State-of-the-art techniques for measuring and monitoring gene level expression rely on messenger RNA (mRNA) extraction and quantification, usually based on the concept of reverse transcription polymerase chain reaction. In this paper, we take adva ...

Ieee2014

NutriChip: Nutrition Analysis meets Microfluidics

Martinus Gijs, Sandro Carrara, Paolo Silacci, Gözen Köklü, Julien Michel Ghaye, Hamideh Jafarpoorchekab, Christine Ruffert, Guy Vergères

This focus article introduces the concept of NutriChip, an integrated microfluidic platform for investigating the potential of the immuno-modulatory function of dairy food. The core component of the NutriChip is a miniaturized artificial human gastrointest ...

Royal Society of Chemistry2013

Image thresholding techniques for localization of sub-resolution fluorescent biomarkers

Giovanni De Micheli, Sandro Carrara, Paolo Silacci, Julien Michel Ghaye, Madhura Avinash Kamat, Guy Vergères

In this article, we explore adaptive global and local segmentation techniques for a lab-on-chip nutrition monitoring system (NutriChip). The experimental setup consists of Caco-2 intestinal cells that can be artificially stimulated to trigger an immune res ...

Wiley-Blackwell2013

The NutriChip project - translating technology into nutritional knowledge

Martinus Gijs, Sandro Carrara, Paolo Silacci, Guy Vergères, Flurina Schwander, Reto Portmann

Advances in food transformation have dramatically increased the diversity of products on the market and, consequently, exposed consumers to a complex spectrum of bioactive nutrients whose potential risks and benefits have mostly not been confidently demons ...

Cambridge University Press2012

NutriChip - A lab-on-a-chip platform to investigate food absorption and underlying immune regulations

Martinus Gijs, Paolo Silacci, Guy Vergères, Flurina Schwander, Reto Portmann

Federation Amer Soc Exp Biol2012

Gastrointestinal Tract on Chip

Martinus Gijs, Paolo Silacci, Hamideh Jafarpoorchekab, Guy Vergères

2012

Nutrichip: an integrated microfluidic system for in vitro investigation of the immunemodulatory function of dairy products

Martinus Gijs, Sandro Carrara, Paolo Silacci, Hamideh Jafarpoorchekab, Guy Vergères, Flurina Schwander

We introduce the concept of the NutriChip, a miniaturized artificial human gastrointestinal tract (GIT) for investigating the potential of immuno-modulatory function of dairy food. More specifically, we are investigating the effect of digested milk on immu ...

2011

Autonomous effects of shear stress and cyclic circumferential stretch regarding endothelial dysfunction and oxidative stress: an ex vivo arterial model

Nikolaos Stergiopoulos, Rafaela Fernandes Da Silva, Paolo Silacci, Tyler Nerton Thacher

Cyclic circumferential stretch and shear stress caused by pulsatile blood flow work in concert, yet are very different stimuli capable of independently mediating endothelial function by modulating eNOS expression, oxidative stress (via production of supero ...

American Soc Mechanical Engineers, Three Park Avenue, New York, Ny 10016-5990 Usa2009

Arterial biomechanics after destruction of cytoskeleton by Cytochalasin D

Nikolaos Stergiopoulos, Sylvain Roy, Paolo Silacci, Tyler Nerton Thacher

Vascular smooth muscle is a major structural element of the arterial wall. We examined the effects of cytoskeleton destruction, after administration of Cytochalasin D, on the biomechanical properties of porcine common carotids. Compared to untreated, maxim ...

2009