Person
David Hacker
Related publications (68)
A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response
David Hacker, Bruno Emanuel Ferreira De Sousa Correia, Florence Pojer, Kelvin Ka Ching Lau
The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a potent adaptive imm ...
2021Analysis of volumetric mass transfer coefficient (k(L)a) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors
Florian Maria Wurm, David Hacker, Likuan Zhu
In this study, the combination of dimensional analysis (DA) and analysis of variance (ANOVA) was used to predict the volumetric mass transfer coefficient (k(L)a) values under different operating conditions for orbitally shaken bioreactors (OSRs) with diffe ...
SPRINGER HEIDELBERG2020Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein
Henning Paul-Julius Stahlberg, David Hacker, Florence Pojer, Dongchun Ni, Kelvin Ka Ching Lau
The human membrane protein Angiotensin-converting enzyme 2 (hACE2) acts as the main receptor for host cells invasion of the new coronavirus SARS-CoV-2. The viral surface glycoprotein Spike binds to hACE2, which triggers virus entry into cells. As of today, ...
2020Chromatin three-dimensional interactions mediate genetic effects on gene expression
David Hacker, Giovanna Ambrosini, Philipp Bucher, Sanjeev Kumar
Studying the genetic basis of gene expression and chromatin organization is key to characterizing the effect of genetic variability on the function and structure of the human genome. Here we unravel how genetic variation perturbs gene regulation using a da ...
2019BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
Bart Deplancke, David Hacker, Bastien Mangeat, Vincent Roland Julien Gardeux, Julie Marie Russeil, Daniel Alpern, Antonio Carlos Alves Meireles Filho
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3 cDNA libraries for dozens of sample ...
2019Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor
Florian Maria Wurm, David Hacker, Dominique Terry Monteil Jr, Likuan Zhu
Orbitally shaken bioreactors (OSRs) are commonly used for the cultivation of mammalian cells in suspension. Here we conducted a three-dimensional computational fluid dynamics (CFD) simulation to characterize the fluid field in the disposable 600-mL orbital ...
Elsevier2018Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes
Florian Maria Wurm, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Likuan Zhu
Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), origina ...
American Chemical Society2017Expression proteomics study to determine metallodrug targets and optimal drug combinations
Paul Joseph Dyson, Kai Johnsson, David Hacker, Claire Samantha Allardyce
The emerging technique termed functional identification of target by expression proteomics (FITExP) has been shown to identify the key protein targets of anti-cancer drugs. Here, we use this approach to elucidate the proteins involved in the mechanism of a ...
2017Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Florian Maria Wurm, David Hacker, Sowmya Balasubramanian
Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...
American Chemical Society2016Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Sowmya Balasubramanian
Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposonspiggyBac, Tol2, and Sleeping Beautyfor their ability to generate c ...
Wiley-Blackwell2016