David Hacker

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Related publications (83)

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

Florian Maria Wurm, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Likuan Zhu

Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), origina ...

American Chemical Society2017

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Sowmya Balasubramanian

Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposonspiggyBac, Tol2, and Sleeping Beautyfor their ability to generate c ...

Wiley-Blackwell2016

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

Florian Maria Wurm, David Hacker, Sowmya Balasubramanian

Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...

American Chemical Society2016

Recombinant protein production from stable mammalian cell lines and pools

David Hacker, Sowmya Balasubramanian

We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...

Elsevier2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Marc Moniatte, Dominique Terry Monteil Jr, Valentin Juvet

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016

Population Variation and Genetic Control of Modular Chromatin Architecture in Humans

Bart Deplancke, David Hacker, Gilles Udin, Sebastian Martin Waszak, Sunil Kumar Raghav, Nouria Hernandez, Alisa Yurovsky, Sarah Thurnheer

Chromatin state variation at gene regulatory elements is abundant across individuals, yet we understand little about the genetic basis of this variability. Here, we profiled several histone modifications, the transcription factor (TF) PU.1, RNA polymerase ...

Elsevier2015

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sowmya Balasubramanian

Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amou ...

American Chemical Society2015

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A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Marc Moniatte, Dominique Terry Monteil Jr, Valentin Juvet

American Chemical Society2016