Publications by Kui Thong Tan | EPFL Graph Search

Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins

Kai Johnsson, Rudolf Hovius, Rudolf Griss, Kui Thong Tan, Silvia Scarabelli

We are introducing a new approach to evaluate cellular uptake of drugs and drug candidates into living cells. The approach is based on converting the protein target of a given class of compounds into a fluorescent biosensor. By measuring the binding of dif ...

Amer Chemical Soc2017

A Semisynthetic Fluorescent Sensor Protein for Glutamate

Kai Johnsson, Luc Reymond, Rudolf Griss, Matthias Albert Brun, Kui Thong Tan

We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor di ...

2012

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Horst Vogel, Kai Johnsson, Luc Reymond, Rudolf Griss, Joachim Piguet, Matthias Albert Brun, Kui Thong Tan, Ruud Peters

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

2011

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

Kai Johnsson, Marlon Hinner, Matthias Albert Brun, Kui Thong Tan

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate Such fluorescent sensor proteins lack. generality, as they require a protein that ...

2009