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Sebastian Maerkl

Related publications (59)

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Transcriptional memory of gene expression enables adaptation to repeated stimuli across many organisms. However, the regulation and heritability of transcriptional memory in single cells and through divisions remains poorly understood. Here, we combined mi ...

CELL PRESS2020

A partially self-regenerating synthetic cell

Sebastian Maerkl, Barbora Lavickova, Nadanai Laohakunakorn

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to r ...

NATURE RESEARCH2020

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Sebastian Maerkl, Laura Sophie Grasemann, Zoe Newell Swank, Barbora Lavickova, Grégoire Michielin, Nadanai Laohakunakorn, Amir Shahein

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

2020

How single-cell immunology is benefiting from microfluidic technologies

Sebastian Maerkl

The immune system is a complex network of specialized cells that work in concert to protect against invading pathogens and tissue damage. Imbalances in this network often result in excessive or absent immune responses leading to allergies, autoimmune disea ...

2020

Microfluidic systems for cancer diagnostics

Sebastian Maerkl, Jose Luis Garcia Cordero

Although not employed in the clinic as of yet, microfluidic systems are likely to become a key technology for cancer diagnostics and prognosis. Microfluidic devices have been developed for the analysis of various biomarkers including circulating tumor cell ...

CURRENT BIOLOGY LTD2020

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Gene-regulatory networks are ubiquitous in nature and critical for bottom-up engineering of synthetic networks. Transcriptional repression is a fundamental function that can be tuned at the level of DNA, protein, and cooperative protein-protein interaction ...

2019

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

Sebastian Maerkl, Barbora Lavickova

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in c ...

AMER CHEMICAL SOC2019

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Cell-based biosensors have great potential to detect various toxic and pathogenic contaminants in aqueous environments. However, frequently they cannot meet practical requirements due to insufficient sensing performance. To address this issue, we investiga ...

NATURE PUBLISHING GROUP2019

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Emulsion drops are often employed as picoliter-sized containers to perform screening assays. These assays usually entail the formation of drops encompassing discrete objects such as cells or microparticles and reagents to study interactions between the dif ...

Nature Publishing Group2018

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The invention relates to a microfluidic device for performing digital analysis of a whole blood sample. ...

2017