Publications by Noelia Laura Bocchio

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Françoise Gisou van der Goot Grunberg, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Theo Lasser, Marcel Leutenegger, Corinne Berclaz, Noelia Laura Bocchio, Claudio Dellagiacoma, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Theo Lasser, Christian Santschi, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Corinne Berclaz, Noelia Laura Bocchio, Matthew Gibson, Joan Goulley

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Nonlinear Correlation Spectroscopy (NLCS)

Theo Lasser, Marcel Leutenegger, Iwan Märki, Jean-Pierre Wolf, Noelia Laura Bocchio, Luigi Bonacina, Stefan Geissbuehler

We present a novel concept for optical spectroscopy called nonlinear correlation spectroscopy (NLCS). NLCS analyses coherent field fluctuations of the second and third harmonic light generated by diffusing nanoparticles. Particles based on noncentrosymmetr ...

American Chemical Society2012

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Theo Lasser, Kai Johnsson, Iwan Märki, Stefan Geissbühler, Noelia Laura Bocchio, Grazvydas Lukinavicius, Claudio Dellagiacoma, Sambashiva Banala

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Reusable Localized Surface Plasmon Sensors Based on Ultrastable Nanostructures

Noelia Laura Bocchio

Nanoparticle arrays created by nanosphere lithography are widely used in sensing applications since their localized surface plasmon resonances are extremely sensitive to changes in the local dielectric environment. A major drawback for any biologically ori ...

Wiley-Blackwell2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010