Publications by Arno Pino Bouwens

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

Theo Lasser, Aleksandra Radenovic, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer, Jochem Deen

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to p ...

2019

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Theo Lasser, Hilal Lashuel, Anne-Laure Mahul Mellier, Marcel Leutenegger, Emrah Bostan, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018

Imaging of cortical structures and microvasculature using extended-focus optical coherence tomography at 1.3 mu m

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag, Damien Nguyen

2018

In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag

SPIE Society of Photo-optical Instrumentation Engineers2018

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Johan Auwerx, Theo Lasser, Laurent Mouchiroud, Paul James Marchand, Arno Pino Bouwens, Vincenzo Sorrentino, Séverine Coquoz, Daniel Pawel Szlag

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Adrien Charles-François Raymond Descloux, Daniel Pawel Szlag, David Nguyen, Jérôme Extermann, Miguel Sison, Séverine Coquoz

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Vincent Khashayar Shamaei, Arno Pino Bouwens, Tristan Bolmont, Daniel Pawel Szlag, Jérôme Extermann, David Nguyen

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Jérôme Extermann, Séverine Coquoz

Optical coherence microscopy (OCM) is an interferometric technique providing 3D images of biological samples with micrometric resolution and penetration depth of several hundreds of micrometers. OCM differs from optical coherence tomography (OCT) in that i ...

2017

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Paul James Marchand, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, David Nguyen, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, Daniel Pawel Szlag, Jérôme Extermann, Joan Goulley

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016