Publications by Carlos Alberto Macias Romero

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

Mapping of real-time morphological changes in the neuronal cytoskeleton with label-free wide-field second-harmonic imaging: a case study of nocodazole

Sylvie Roke, Carlos Alberto Macias Romero, Pascal Jourdain, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

We demonstrate the use of wide-field high-throughput second-harmonic (SH) microscopy for investigating cytoskeletal morphological changes on the single-cell level. The method allows for real-time, in vitro, label-free measurements of cytoskeletal changes t ...

SPIE-Intl Soc Optical Eng2019

Optical imaging of surface chemistry and dynamics in confinement

Sylvie Roke, Carlos Alberto Macias Romero, Halil Ibrahim Okur, Igor Nahálka

ACS2018

Optical imaging of surface chemistry and dynamics in confinement

Sylvie Roke, Carlos Alberto Macias Romero, Halil Ibrahim Okur, Igor Nahálka

We imaged the interfacial structure and dynamics of water in a microscopically confined geometry in three dimensions and on millisecond time scales with a 3D structurally illuminated wide-field second harmonic microscope. Image contrast reports on the orie ...

American Association for the Advancement of Science2017

Characterization of the interface of binary mixed DOPC:DOPS liposomes in water: The impact of charge condensation

Sylvie Roke, Carlos Alberto Macias Romero, Cornelis Ulrich Lütgebaucks

Solutions of liposomes composed of binary mixtures of anionic dioleoylphosphatidylserine (DOPS) and zwitterionic dioleoylphosphatidylcholine (DOPC) are investigated with label-free angle-resolved (AR) second harmonic scattering (SHS) and electrophoretic mo ...

2017

Wide-field medium-repetition-rate multiphoton microscopy reduces photodamage of living cells

Sylvie Roke, Carlos Alberto Macias Romero, Siyuan Wang, Vitalijs Zubkovs

Demands of higher spatial and temporal resolutions in linear and nonlinear imaging keep pushing the limits of optical microscopy. We showed recently that a multiphoton microscope with 200 kHz repetition rate and wide-field illumination has a 2–3 orders of ...

2016

Electrolytes induce long-range orientational order and free energy changes in the H-bond network of bulk water

Michele Ceriotti, Sylvie Roke, Carlos Alberto Macias Romero, Yixing Chen, Halil Ibrahim Okur, David Mark Wilkins, Gabriele Tocci, Chung-Wen Liang, Nikolaos Gomopoulos

Electrolytes interact with water in many ways: changing dipole orientation, inducing charge transfer, and distorting the hydrogen-bond network in the bulk and at interfaces. Numerous experiments and computations have detected short-range perturbations that ...

American Association for the Advancement of Science (AAAS)2016

Device and method for measuring and imaging second harmonic and multi-photon generation scattered radiation

Sylvie Roke, Carlos Alberto Macias Romero

Embodiments of the subject invention relate to a method and apparatus for performing measurements using multiphoton or second harmonic generation (SHG) scattered radiation from a sample including a turbid (scattering) medium includes providing a beam of la ...

2015

Confocal polarization imaging in high-numerical-aperture space

Carlos Alberto Macias Romero

In this work we describe theoretical and experimental physical aspects of high-resolution imaging polarimetry and its application to polarization-multiplexed encoding. We theoretically demonstrate that it is possible to resolve the orientation of two fixed ...

Optical Soc Amer2014

Probing Rotational and Translational Diffusion of Nanodoublers in Living Cells on Microsecond Time Scales

Sylvie Roke, Aleksandra Radenovic, Carlos Alberto Macias Romero, Fabrizia Dutto, Vitalijs Zubkovs, Marie Eve Pascale Didier, Lucas Delannoy

Nonlinear microscopes have seen an increase in popularity in the life sciences due to their molecular and structural specificity, high resolution, large penetration depth, and volumetric imaging capability. Nonetheless, the inherently weak optical signals ...

American Chemical Society2014