Person

Azat Sharipov

This person is no longer with EPFL

Related publications (6)

Multi-plane super-resolution microscopy

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

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Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, supe ...

Public Library of Science2016

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Marcel Leutenegger, Daniel Pawel Szlag, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

2014

Multiplane 3D superresolution optical fluctuation imaging

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Azat Sharipov, Stefan Geissbuehler

By switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical fluctuation imaging ...

2013

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