Publications by Tomas Lukes | EPFL Graph Search

Open-source microscope add-on for structured illumination microscopy

Theo Lasser, Matthias Lütolf, Aleksandra Radenovic, Andrew Charles Oates, Georg Fantner, John McKinney, Nathan Cyprien Arsène Ronceray, Olivier François Venzin, Antonius Chrisnandy, Prabhu Prasad Swain

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to ...

Nature Portfolio2024

Smoothness correction for better SOFI imaging

Tomas Lukes

Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain an improved spat ...

NATURE RESEARCH2021

Spectral cross-cumulants for multicolor super-resolved SOFI imaging

Theo Lasser, Aleksandra Radenovic, Marcel Leutenegger, Kristin Stefanie Grussmayer, Tomas Lukes, Adrien Charles-François Raymond Descloux

Super-resolution opticalfluctuation imaging provides a resolution beyond the diffraction limitby analysing stochasticfluorescencefluctuations with higher-order statistics. Usingnthorderspatio-temporal cross-cumulants the spatial resolution and the sampling ...

Nature Research2020

Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation Imaging

Theo Lasser, Aleksandra Radenovic, Tomas Lukes, Kristin Stefanie Grussmayer

Most diffraction-unlimited super-resolution imaging critically depends on the switching of fluorophores between at least two states, often induced using intense laser light and specialized buffers or UV radiation. Recently, so-called self-blinking dyes tha ...

AMER CHEMICAL SOC2020

High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Theo Lasser, Aleksandra Radenovic, Tomas Lukes, Vytautas Navikas, Adrien Charles-François Raymond Descloux

Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, ...

2020

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

Theo Lasser, Aleksandra Radenovic, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer, Jochem Deen

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to p ...

2019

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Michaël Unser, Daniel Sage, Thanh-An Michel Pham, Tomas Lukes, Seamus John Holden, Anna Archetti, Thomas Pengo, Ricardo Henriques, Silvia Colabrese, Anurag Agrawal

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effo ...

2019

Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction

Tomas Lukes, Jakub Pospisil

Background: Structured illumination microscopy (SIM) is a family of methods in optical fluorescence microscopy that can achieve both optical sectioning and super-resolution effects. SIM is a valuable method for high-resolution imaging of fixed cells or tis ...

Oxford University Press (OUP)2019

Influence of Artificially Generated Interocular Blur Difference on Fusion Stability Under Vergence Stress

Tomas Lukes

The stability of fusion was evaluated by its breakage when interocular blur differences were presented under vergence demand to healthy subjects. We presumed that these blur differences cause suppression of the more blurred image (interocular blur suppress ...

2019

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Theo Lasser, Hilal Lashuel, Anne-Laure Mahul Mellier, Marcel Leutenegger, Emrah Bostan, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018