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Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells
Related publications (88)
Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy
Kai Johnsson, Lu Wang, Aleksandar Salim, Michelle Susanne Frei
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spa ...
AMER CHEMICAL SOC2019Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting
The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein assemblies and cellu ...
2019Improving 3D MA-TIRF Reconstruction with Deconvolution and Background Estimation
Emmanuel Emilien Louis Soubies, Sébastien Schaub
Total internal reflection fluorescence microscopy (TIRF) produces 2D images of the fluorescent activity integrated over a very thin layer adjacent to the glass coverslip. By varying the illumination angle (multi-angle TIRF), a stack of 2D images is acquire ...
IEEE2019Design and Synthesis of Gatekeeper Coated Dendritic Silica/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy
Saba Rezakhani, Samira Malekmohammadi
New dendritic silica/titania mesoporous nanoparticles (DSTNs) loaded with curcumin (CUR) were synthesized and coated with polyethylenimine-folic acid groups (PEI-FA) for an ultrasound (US)-triggered drug release and combined chemo-sonodynamic therapy. The ...
2019Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
Photoswitchable mols. have multiple applications in the phys. and life sciences because their properties can be modulated with light. Fluxional mols., which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching beha ...
Springer2019Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)
Ardemis Anoush Boghossian, Benjamin Paul Johanès Gabriel Lambert, Alessandra Antonucci, Vitalijs Zubkovs
Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...
2018Visual Analytics of Single Cell Microscopy Data Using a Collaborative Immersive Environment
Sarah Irene Brutton Kenderdine
Understanding complex physiological processes demands the integration of diverse insights derived from visual and quantitative analysis of bio-image data, such as microscopy images. This process is currently constrained by disconnects between methods for i ...
ASSOC COMPUTING MACHINERY2018Coherent Imaging of Cellular Dynamics
Mitochondrial dynamics refers to the processes of fusion, fission, and transport that aid mitochondria in accomplishing their many roles; including ATP production, oxygen sensing, and homeostasis. Due to their involvement in numerous essential cellular act ...
EPFL2017Investigating Focal Adhesion Substructures by Localization Microscopy
Aleksandra Radenovic, Daniel Sage, Lely Feletti, Hendrik Georges Deschout
Cells rely on focal adhesions (FAs) to carry out a variety of important tasks, including motion, environmental sensing, and adhesion to the extracellular matrix. Although attaining a fundamental characterization of FAs is a compelling goal, their extensive ...
Elsevier2017Nanowire-Aperture Probe: Local Enhanced Fluorescence Detection for the Investigation of Live Cells at the Nanoscale
Anna Fontcuberta i Morral, Esther Alarcon Llado
Fluorescence microscopy has tackled many of the burning questions in cellular biology. Probing low-affinity cellular interactions remains one of the major challenges in the field to better understand cellular signaling. We introduce a novel approach the na ...
Amer Chemical Soc2016