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Micro- and nanostructured devices for investigating biomolecular interactions

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Organohalide respiration: variations on a common theme

Christof Holliger, Julien Maillard, Mathilde Stéphanie Willemin, Lorenzo Cimmino

Organohalide respiration (OHR) is a bacterial anaerobic respiratory metabolism that makes use of halogenated organic compounds as terminal electron acceptors. While organohalogens have been initially thought to be mainly from anthropogenic origin, thousand ...

2021

Influence of Detergent and Lipid Composition on Reconstituted Membrane Proteins for Structural Studies

Mohammed Mouhib

Membrane proteins are frequently reconstituted in different detergents as a prerequisite to create a phospholipid environment reminiscent of their native environment. Different detergent characteristics such as their chain length and bond types could affec ...

AMER CHEMICAL SOC2021

Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in par ...

2021

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

EPFL2021

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The pceABCT gene cluster from Dehalobacter restrictus is one model system for tetrachloroethene (PCE) respiration. In the present study we aim to decipher the stoichiometry of the pceABCT gene products and the composition of PceA-containing membrane-bound ...

2021

Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporat ...

2020

Live-cell lipid biochemistry reveals a role of diacylglycerol side-chain composition for cellular lipid dynamics and protein affinities

Milena Maria Schuhmacher, Pavel Barahtjan

Every cell produces thousands of distinct lipid species, but insight into how lipid chemical diversity contributes to biological signaling is lacking, particularly because of a scarcity of methods for quantitatively studying lipid function in living cells. ...

2020

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A fundamental question of eukaryotic cell biology is how membrane organelles are organised and interact with each other. Cell biologists address these questions by characterising the structural features of membrane compartments and the mechanisms that coor ...

WILEY2020

Polarly Localized EccE(1) Is Required for ESX-1 Function and Stabilization of ESX-1 Membrane Proteins in Mycobacterium tuberculosis

Stewart Cole, Claudia Sala, Ming Zhang, Jérémie Piton, Paloma Julia Soler Arnedo

Mycobacterium tuberculosis is a slow-growing intracellular bacterium with the ability to induce host cell death and persist indefinitely in the human body. This pathogen uses the specialized ESX-1 secretion system to secrete virulence factors and potent im ...

AMER SOC MICROBIOLOGY2020

Multi-color Molecular Visualization of Signaling Proteins Reveals How C-Terminal Src Kinase Nanoclusters Regulate T Cell Receptor Activation

Juliette Griffie

Elucidating the mechanisms that controlled T cell activation requires visualization of the spatial organization of multiple proteins on the submicron scale. Here, we use stoichiometrically accurate, multiplexed, single-molecule super-resolution microscopy ...

CELL PRESS2020