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Detection efficiency in total internal reflection fluorescence microscopy
Related publications (34)
Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Yichen Feng
Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...
Spie-Soc Photo-Optical Instrumentation Engineers2024Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy
Aleksandra Radenovic, Georg Fantner, Vytautas Navikas, Adrien Charles-François Raymond Descloux, Samuel Mendes Leitão, Kristin Stefanie Grussmayer
High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...
2021Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization
Michael Stefan Daniel Liebling, Christian Jaques
Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...
2020Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy
Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...
EPFL2020Improving 3D MA-TIRF Reconstruction with Deconvolution and Background Estimation
Emmanuel Emilien Louis Soubies, Sébastien Schaub
Total internal reflection fluorescence microscopy (TIRF) produces 2D images of the fluorescent activity integrated over a very thin layer adjacent to the glass coverslip. By varying the illumination angle (multi-angle TIRF), a stack of 2D images is acquire ...
IEEE2019Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting
The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein assemblies and cellu ...
2019Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures
Super-resolution fluorescence microscopy is widespread, owing to its demonstrated ability to resolve dynamical processes within cells and to identify the structure and position of specific proteins in the interior of protein complexes. Nowadays, subcellula ...
EPFL2019Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)
Ardemis Anoush Boghossian, Benjamin Paul Johanès Gabriel Lambert, Alessandra Antonucci, Vitalijs Zubkovs
Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...
2018Nanopatterning of a Stimuli-Responsive Fluorescent Supramolecular Polymer by Thermal Scanning Probe Lithography
Jürgen Brugger, Samuel Zimmermann
The miniaturization of nanometer-sized multicolor fluorescent features is of continuous significance for counterfeit security features, data storage, and sensors. Recent advances in engineering of stimuli-responsive supramolecular polymeric materials that ...
2017Intense fluorescence of Au-20
Harald Brune, Wolfgang Harbich, Chongqi Yu
Ligand-protected Au clusters are non-bleaching fluorescence markers in bio- and medical applications. Here we show that their fluorescence can be an intrinsic property of the Au cluster itself. We find a very intense and sharp fluorescence peak located at ...
American Institute of Physics2017