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The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...
This review article summarizes efforts to use nitrous oxide (N2O, ‘laughing gas’) as a reagent in synthetic chemistry. The focus will be on reactions which are carried out in homogeneous solution under (relatively) mild conditions. First, the utilization o ...
An in-situ modulated photocurrent technique to study kinetics of dye adsorption and to determine binding constants of dye molecules on the TiO 2 surface is presented. An AC-photocurrent signal is measured, when a TiO2 electrode is in contact with the dye b ...
Single particle tracking can reveal dynamic information at the scale of single molecules in living cells but thus far has been limited either in the range of potential protein targets or in the quality and number of tracks attainable. We demonstrate a new ...
Mesoporous silicates (MPS) provide a means of immobilizing proteins and enzymes in a stable environment while retaining physiological function. A systematic methodology of assessing the potential of immobilising a given protein on to MPS has been developed ...
Identification of proteins in 3D maps of cells is a main challenge in structural cell biology. For light microscopy (LM) clonable reagents such as green fluorescent protein represented a real revolution and equivalent reagents for transmission electron mic ...
Chronic myeloid leukemia is characterized by a reciprocal chromosomal translocation between chromosome 9 and 22, resulting in the expression of the Bcr-Abl oncoprotein. Despite the great improvement in patient survival using tyrosine kinase inhibitors (TKI ...
The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...
Scanning electrochemical microscopy (SECM) has developed into an excellent and versatile technique to image heterogeneous chemical reactivities on a surface. Therefore SECM applications cover a wide range of different fields such as biology and forensic sc ...
We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative ...
