Controlled entrapment of cells in hydrogels on chip and cell sorting by dielectrophoresis using liquid electrodes
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We propose a model to determine the influence of different cell properties, such as size, membrane capacitance and cytoplasm conductivity, on the impedance spectrum as measured in a microfabricated cytometer. A dielectric sphere of equivalent complex permi ...
The Off-Gel™ technology was recently described for protein fractionation in a solution placed on top of an immobilized pH gradient gel. In addition, this process was found to remove salts from the biological samples to analyze. This desalting effect is stu ...
The ultrafast evolution of the electric field within bacteriorhodopsin was measured by monitoring the absorption changes of a tryptophan residue after excitation of retinal. The Trp absorption decreases within the first 200 femtoseconds and then recovers o ...
The use of interdigitated electrodes of nanometer scale establishes a broad field of new applications particularly in biotechnology. It allows to create electric field gradients at submicrometer scale. The goal of the project is the fabrication and charact ...
The Off-Gel™ technology is an isoelectric focusing (IEF) fractionation method which enables the purification of one or several proteins in a solution placed on an immobilised pH gradient (IPG) gel. The present thesis is devoted to its characterisation and ...
An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents we ...
In this thesis, a new microfabricated flow cytometer for single cell discrimination based on dielectric spectroscopy is presented. Integrated tools for rapid in-flow cell handling are also demonstrated. Signal amplification and analysis as well as the chip ...
We study the dielectric susceptibility of liquid hydrogen chloride at a temperature of 313 K using ab initio molecular dynamics evolving, in a homogeneous electric field. For an evolution in absence of electric field, the calculated neutron structure facto ...
Homogeneous electric fields are treated within density functional calculations with periodic boundary conditions. A nonlocal energy functional depending on the applied field is used within an ab initio molecular dynamics scheme. The reliability of the meth ...
Electrostatically driven MEMS devices commonly operate with electric fields as high at 108 V/m applied across the dielectric between electrodes. Even with the best mechanical design, the electrical design of these devices has a large impact both on perform ...
Institute of Electrical and Electronics Engineers2004