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Single-molecule Forster resonance energy transfer (FRET) and photoinduced electron transfer (PET) have developed into versatile and complementary methods for probing distances and dynamics in biomolecules. Here we show that the two methods can be combined ...
Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact prob ...
Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...
To ensure efficacy but avoid toxicity, many drugs require monitoring of their concentrations in the patient’s blood during therapy. Currently, therapeutic drug monitoring (TDM) is done in diagnostic laboratories that need trained personnel and expensive in ...
Fluorescence resonance energy transfer (FRET) efficiency measurements based on acceptor photobleaching of yellow fluorescent protein (YFP) are affected by the fact that bleaching of YFP produces a fluorescent species that is detectable in cyan fluorescent ...
Background: In mammals, ABCB1 constitutes a cellular "first line of defense" against a wide array of chemicals and drugs conferring cellular multidrug or multixenobiotic resistance (MDR/MXR). We tested the hypothesis that an ABCB1 ortholog serves as protec ...
Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...
Natural and synthetic purine derivatives such as caffeine, theophylline, 6-mercaptopurine and 8-chlorotheophylline are important drugs. Due to the structural similarity of these compounds, it is intrinsically difficult to prepare chemosensors for their sel ...
Digital image processing and epi-fluorescence microscopy provide one of the main and basic tools for living biological cells analysis and studying. Developing, testing and comparing those image processing methods properly require the use of a controlled en ...
An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescence vers ...
