Publications related to Chromosomal Number Aberrations and Transformation in Adult Mouse Retinal Stem Cells In Vitro

Genotypic features of lentivirus transgenic mice

Didier Trono, Denis Duboule, Felix Naef, Frédéric Schütz, Marc-Olivier Sauvain

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Lara Buscemi Estefanell, Pierre Jean Arnould Wipff

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet, Nicolas Jaccard

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

Heterogeneity in multistage carcinogenesis and mixture modeling

Stephan Morgenthaler, Sandro Gsteiger

Carcinogenesis is commonly described as a multistage process, in which stem cells are transformed into cancer cells via a series of mutations. In this article, we consider extensions of the multistage carcinogenesis model by mixture modeling. This approach ...

BioMed Central2008

Genetic engineering of embryonic stem cells

David Michael Suter

We describe a novel generation oflentiviral vectors that are particularly well suited for work with embryonic stem cells. The possibility of selecting cell lines with antibiotics and the rapid insertion of any combination of promoters and genes of interest ...

Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2007

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Bo Song, Steve Comby

The homoditopic ligand

H_2L^{C3}

has been designed to form neutral triple-stranded bimetallic helicates of overall composition [

Ln_2(L^{C3})_3

]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration whi ...

2007

From material flow analysis to material flow management Part I: social sciences modeling approaches coupled to MFA

Claudia Rebeca Binder Signer

This paper presents social sciences modeling approaches (SSMA) that have been coupled to material flow analyses in order to support management of material flows. The presented literature review revealed that the large share of these approaches stem from ec ...

2007

Stem cells and their niche: a matter of fate

Olaia Maria Naveiras Torres-Quiroga

Embryonic stem cells provide an in vitro model for developmental biologists to study cell fate decisions during ontogenesis, while somatic stem cells allow physiologists to understand tissue homeostasis in the adult. The behavior of stem cells is dependent ...

Springer Verlag2006

Two-dimensional microimpedance imaging for cell culture monitoring

Olof Pontus Linderholm

This thesis presents the fabrication and design of an impedance imaging sensor, and its application to monitoring in-vitro skin culture. Three-dimensional tissue cultures are becoming increasingly important in biological and medical research. There is, how ...

EPFL2006

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, David Hacker, Madiha Derouazi, Martin Bertschinger

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Chromosomal Number Aberrations and Transformation in Adult Mouse Retinal Stem Cells In Vitro

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Genotypic features of lentivirus transgenic mice

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Heterogeneity in multistage carcinogenesis and mixture modeling

Genetic engineering of embryonic stem cells

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

From material flow analysis to material flow management Part I: social sciences modeling approaches coupled to MFA

Stem cells and their niche: a matter of fate

Two-dimensional microimpedance imaging for cell culture monitoring

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Genetic engineering of embryonic stem cells

Heterogeneity in multistage carcinogenesis and mixture modeling

Genotypic features of lentivirus transgenic mice

From material flow analysis to material flow management Part I: social sciences modeling approaches coupled to MFA

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Stem cells and their niche: a matter of fate

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Two-dimensional microimpedance imaging for cell culture monitoring

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines