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Sub-Rayleigh resolution by phase imaging

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In vivo assessment of myelination by phase imaging at high magnetic field

Rolf Gruetter, José Pedro Rebelo Ferreira Marques, Rajika Maddage

The present study evaluated the potential of using the phase of T-2* weighted MR images to characterize myelination during brain development and pathology in rodents at 9.4 T. Phase contrast correlated with myelin content assessed by histology and suggests ...

Elsevier2012

Complex index refraction tomography with sub ?/6-resolution

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte

The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...

2011

Beyond the lateral resolution limit by phase imaging

Christian Depeursinge, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a theory stating how to overcome the classical Rayleigh-resolution limit. It is based upon a new resolution criterion in phase of coherent imaging process and its spatial resolution is thought to be only SNR limited. Recently, the experimental o ...

SPIE2011

Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy

Giorgio Margaritondo

Background: Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptak ...

BioMed Central2011

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

Nicolas Pavillon

We present in this PhD thesis work various applications of digital holographic microscopy (DHM), an imaging technique based on coherent illumination which enables the recovery of the full complex wavefront, i.e. the amplitude and phase of a wave field whic ...

EPFL2011

Beyond the lateral resolution limit by phase imaging

Christian Depeursinge, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a theory to extend the classical Abbe resolution limit by introducing a spatially varying phase into the illumination beam of a phase imaging system. It allows measuring lateral and axial distance differences between point sources to a higher ac ...

2011

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Digital Holographic Microscopy (DHM) has been used to investigate the spontaneous cell membrane fluctuations (CMF) of the Red Blood Cell. DHM as an interferometric technique is able to accurately provide the wavefront deformation induced by a transparent s ...

SPIE2010

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Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

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Recent studies at high magnetic fields using the phase of gradient-echo MR images have shown the ability to unveil cortical substructure in the human brain. To investigate the contrast mechanisms in phase imaging, this study extends, for the first time, ph ...

Elsevier2009

Digital holographic microscopy at fundamental and second harmonic wavelengths

Christian Depeursinge, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering of the complex diffraction wavefields from whi ...

SPIE2009