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The development and application of methods quantifying the metabolic fluxes such as Flux Balance Analysis (FBA) has been one of the driving forces behind the successful growth of metabolic engineering. However, applications of FBA have been limited by the ...
Xylose fermentation from lignocellulosic material using recombinant yeast cells is a promising resource for efficient ethanol production as a future sustainable energy supply. Experimental approaches emphasize the engineering of xylose-utilizing recombinan ...
Study of a metabolic network with respect to the sensitivity of the metabolite concentrations and the metabolic fluxes to genetic, biochemical, and environmental variations requires knowledge of the kinetic properties of the enzymes in the network. Experim ...
Recently the function of the sirtuin family, named after their homology to the Saccharomyces cerevisiae gene silent information regulator 2 (Sir2), has received a lot of attention, as their beneficial impact on longevity was linked to their effects on meta ...
Advancement of genomic research and anal. techniques enables an in-depth understanding of individual components in the cellular metab. The crit. subject is the integration of massive exptl. details and the quantification of the contributions of individual ...
A multitude of metabolic regulations occur in yeast, particularly under dynamic process conditions, such as under sudden glucose excess. However, quantification of regulations and classification of yeast strains under these conditions have yet to be elucid ...
Achieving practical biotechnol. processes for the prodn. of fine chems. requires optimization of metabolic fluxes. Attempts to alter the fluxes through a given pathway may be frustrated by compensatory changes in coupled pathways or by unexpected effects s ...
In vivo 13C NMR spectroscopy has the unique capability to measure metabolic fluxes noninvasively in the brain. Quantitative measurements of metabolic fluxes require analysis of the 13C labeling time courses obtained experimentally with a metabolic model. T ...
Thermodynamic feasibility analysis (TFA) has been used as a toot capable of providing additional constraints to the mass balance-based methods of analysis of Metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in w ...
Mathematical modeling is an indispensable tool for research and development in biotechnology and bioengineering. The formulation of kinetic models of biochemical networks depends on knowledge of the kinetic properties of the enzymes of the individual react ...