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Fluidic microstructuring of alginate hydrogels for the single cell niche

Jürgen Brugger, Philippe Renaud, Thomas Michael Braschler, Kristopher Pataky, Ana Valero, Ludovica Colella
2010
Journal paper
Abstract

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.

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