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Development of high content image-based cell viability assays
Related publications (37)
Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics
Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...
EPFL2024Label-free plasmonic microarray for multiplexed analysis of cells and tumor organoids
Investigating the dynamic activities of protein expression and signaling in living organisms is a crucial focus of intense research aimed at elucidating the processes that underlie disease progression and improving treatments and drug development. Resolvin ...
EPFL2023Multitarget-Directed Gallium(III) Tris(acyl-pyrazolonate) Complexes Induce Ferroptosis in Cancer Cells via Dysregulation of Cell Redox Homeostasis and Inhibition of the Mevalonate Pathway
A series of Ga(Q(n))(3) coordination compounds have been synthesized, where HQ(n) is 1-phenyl-3-methyl-4-RC(=O)-pyrazolo-5-one. The complexes have been characterized through analytical data, NMR and IR spectroscopy, ESI mass spectrometry, elemental analysi ...
AMER CHEMICAL SOC2023Generating macrocyclic inhibitors of protein-protein interactions
Protein-protein interactions (PPIs) play important roles in many diseases and their modulation is an attractive strategy for developing new therapeutics. However, the relatively large and often flat binding surfaces of PPIs makes the development of inhibit ...
EPFL2022The pro-apoptotic properties of a phytonutrient rich infusion of A. cherimola leaf extract on AML cells
Natalia Gasilova, Laure Menin, Rita Sarkis, Maria Younes
Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds' or leaves' extracts. The present study investigates the antiproliferative and antitumor activity of Annona cherimola aqueous leaf (AAL) extract/in ...
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER2021The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...
EPFL2019The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines
Background The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Le ...
BMC2019Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Olaia Maria Naveiras Torres-Quiroga, George Coukos, Mukul Girotra, Nicola Vannini, Alexandre Harari
A fine balance of quiescence, self-renewal, and differentiation is key to preserve the hematopoietic stem cell (HSC) pool and maintain lifelong production of all mature blood cells. In recent years cellular metabolism has emerged as a crucial regulator of ...
2019Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux
Françoise Gisou van der Goot Grunberg, Christopher Claude Giuseppe Tremblay, Patrick Alain Sandoz
Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell’s refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mamm ...
2019Single live cell monitoring of protein turnover reveals intercellular variability and cell cycle dependence of degradation rates
Cells need to be able to precisely control their proteome composition in order to maintain homeostasis. This is of particular relevance in fast dividing cells, such as embryonic stem cells, since their global protein levels need to be doubled at each divis ...
EPFL2018