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Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging

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Holographic Second Harmonic Generation Microscopy

Etienne Shaffer

This dissertation is dedicated to the application of digital holography methods to second harmonic generation microscopy, and to the development of the resulting imaging technique: holographic second harmonic generation microscopy. Second harmonic generati ...

EPFL2011

Holographic second harmonic generation (SHG) microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

The coherent nature of second harmonic generation (SHG) makes possible retrieval of both its amplitude and its phase, by use of a proper phase-sensitive imaging technique such as digital holographic SHG microscopy. In addition to retrieving both amplitude ...

2011

Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh, Grégoire Pierre Jean Laporte

We demonstrate imaging through a turbid layer by using digital phase conjugation of the second harmonic field radiated from a beacon nanoparticle. We show that the phase-conjugated focus can be displaced from its initial position by illuminating the same r ...

2010

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We demonstrate focusing coherent light on a nanoparticle through turbid media based on digital optical phase conjugation of second harmonic generation (SHG) field from the nanoparticle. A SHG active nanoparticle inside a turbid medium was excited at the fu ...

2010

Digital holography for second harmonic microscopy

Christian Depeursinge, Etienne Shaffer

Quantitative phase images make digital holographic microscopy (DHM) an excellent instrument for metrological, but also for biological applications, where it can reveal deformations and morphological details at ultrahigh resolution in the order of a few nan ...

SPIE2010

Method and apparatus for retrieval of amplitude and phase of nonlinear electromagnetic waves

Christian Depeursinge, Etienne Shaffer

The present invention discloses a method and its associated apparatus to retrieve the amplitude and, especially, the phase of nonlinear electromagnetic waves. The application field of the present invention is optical imaging. A sample is probed by coherent ...

2010

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A nonlinear optical plasmonic core-shell nanocavity is demonstrated as an efficient, subwavelength coherent light source through second-harmonic generation. The nonlinear optical plasmonic nanocavity incorporates a noncentrosymmetric medium, which utilizes ...

2010

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Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocry ...

Optical Society of America2009

Harmonic holography for three-dimensional cellular imaging

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocr ...

2009

Harmonic holographic microscopy with circularly polarized excitation

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We have developed a luminescent marker using the second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time ...

2009