Autofluorescence detection of tumors in the human lung - spectroscopical measurements in situ, in an in vivo model and in vitro
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Background. To optimize photodynamic therapy (PDT) and photodetection of cancer, two important variables that must be considered are the uptake of the dye and the dye contrast between normal and neoplastic tissue after injection. Methods. To study these va ...
A method is proposed for the sepn. of overlapping multiplets or substructure within multiplets in two-dimensional spectra. The issue of the recognition and measurement of homonuclear couplings in arbitrary mols. was discussed. The overlapping cross-peak mu ...
To optimize photodynamic therapy (PDT) and photodetection of cancer with second-generation photosensitizers, knowledge of important variables such as the uptake of the dye and the dye contrast between normal and tumoral tissue after injection is necessary. ...
Passive spin filters are combined with pure in-phase correlation spectroscopy (PICSY) to obtain SIS-(p,q)-PICSY expts., where p and q are the nos. of passive spins that are simultaneously or sequentially inverted during the evolution period and that are co ...
It is shown how amplitude-modulated radio-frequency pulses optimized for selective inversion of magnetization can be applied to static powder samples with inhomogeneously broadened spectra. Selective one-dimensional methods can in principle provide informa ...
Golden Syrian hamsters are evaluated as an animal model for phototherapy of early squamous cell carcinomas of the mucosa of the upper aerodigestive tract, the esophagus and the tracheobronchial tree. Carcinomas of this type are induced on the hamster cheek ...
In vivo spectrofluorometric analysis represents a tool to obtain information about fluorophore distribution in tissue. Based on a Peltier-cooled CCD we designed a fluorescence excitation and emission spectrograph which allows to obtain tissue spectra endos ...
To improve the detectability of tumors by light-induced fluorescence, the use of monoclonal antibodies (MoAb) as carriers of fluorescent molecules was studied. As a model for this approach, the biodistribution of an anticarcinoembryonic antigen (CEA) MoAb ...
In 2-dimensional correlation spectra of strongly coupled spin systems obtained with the z-filtered COSY technique, the amplitudes of cross and diagonal peaks obey simple rules. They are simply proportional to the product of the amplitudes of the correspond ...
The authors have recently shown that immunophotodetection of human colon carcinomas in nude mice and in patients is possible by using anti-carcinoembryonic antigen monoclonal antibodies (MAb) coupled to fluorescein. The most common clin. application of pho ...