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Second harmonic phase microscopy of collagen fibers

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Second harmonic generating (SHG) Nanoprobes: A new tool for biomedical imaging

Demetri Psaltis, Ye Pu

Cornea functions as an outermost lens and plays an important role in vision. For cornea diagnosis and treatment, a microscopic imaging system with cellular resolution and high eye safety is strongly desired. Recently, the cell morphology of corneal epithel ...

2009

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocry ...

Optical Society of America2009

Harmonic holography for three-dimensional cellular imaging

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocr ...

2009

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We have developed a luminescent marker using the second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time ...

2009

Harmonic Holographic Microscopy Using Nanoparticles as Probes for Three-Dimensional Cell Imaging

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We demonstrate the three-dimensional imaging capability of harmonic holographic microscopy by using the second harmonic generation from BaTiO3 nanoparticles as the signal. Three-dimensional distributions of the BaTiO3 nanoparticles in biological cells are ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

The process of second harmonic generation (SHG) has a unique property of forming a sharp optical contrast between noncentrosymmetric crystalline materials and other types of material, which is a highly valuable asset for contrast microscopy. The coherent s ...

Optical Society of America2008

Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Pierre Marquet, Jonas Kühn, Elena Cano

Digital holography microscopy (DHM) is an optical microscopy technique which allows recording non-invasively the phase shift induced by living cells with nanometric sensitivity. Here, we exploit the phase signal as an indicator of dry mass (related to the ...

SPIE2008

A study of the microstructural evolution of Zircaloy-4 (Zy-4) during hot extrusion has been performed on a small scale device mounted on a standard MTS machine. Before extrusion the billets present a Wind-manstätten lamellar microstructure. Additional data ...

2008

Tunable nanowire nonlinear optical probe

Aleksandra Radenovic

One crucial challenge for subwavelength optics has been the development of a tunable source of coherent laser radiation for use in the physical, information and biological sciences that is stable at room temperature and physiological conditions. Current ad ...

2007

The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...

EPFL2007