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k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Related publications (35)

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Continuous and label-free toxicity screening of human hepatocytes on chip reveals frequency-dependant impedance profiles

Philippe Renaud, Robert Meissner, Bilge Eker

We present an electrical impedance based biosensor on a microfluidic cell culture platform that enables label-free and re- al-time investigation of drug toxicity-induced effects on hepatocytes. The temporal evolution of the impedance at different frequenci ...

2011

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Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been explored. From o ...

2010

Microfluidics-generated immobilized biomolecule gradients on hydrogels

Matthias Lütolf, Stefan Kobel, Steffen Cosson

Extra-cellular matrix (ECM) tethered morphogen gradients play an important role in tissue development and regeneration. There is currently only a limited set of model systems which can mimic these gradients in an artificial ECM-like environment. We have de ...

The Chemical and Biological Microsystems Society2008

Device for the in vitro culture of cells comprising: a planar deformable substrate ( 3 ) with a culture surface ( 4 ), substrate deforming means for modifying the size of said culture surface ( 4 ), characterized by the fact that it furthermore comprises: ...

2008

We present a novel, inexpensive, and fast microimpedance tomography system for two-dimensional imaging of cell and tissue cultures. The system is based on four-electrode measurements using 16 planar microelectrodes (5 mum x 4 mm) integrated into a culture ...

2008

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We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of ...

2007

Characterization of Fibra-Cel® packed-bed bioreactors for the culture of CHO cells

Frédéric Meuwly

Today, more than 30 products using a manufacturing process based on mammalian cell culture have been approved for human therapy. Most of these products are currently supplied with stirred tank bioreactors operated in batch or fed-batch mode. However, the b ...

EPFL2006

Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities

Nico de Rooij

In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4 +) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or different ...

2006

3D morphology of cell cultures: a quantitative approach using micrometer synchrotron light tomography

Jeffrey Alan Hubbell, Randoald Müller

Current issues in both tissue engineering and cell biology deal with cell behavior extensively in 3D. Here, we explore synchrotron radiation micro-computed tomography as a tool for morphological characterization of such 3D cellular constructs, providing mi ...

2005

Modular microsystem for epithelial cell culture and electrical characterisation

Martinus Gijs, Abdeljalil Sayah, Serge Hediger

We have realised a microsystem for the culture and electrical characterisation of epithelial cell layers for cell-based diagnostic applications. The main goal of this work is to achieve both cell culture and impedimetric and potentiometric characterisation ...

2001