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High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

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Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Quantitative phase imaging in biomedicine

Christian Depeursinge

Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobl ...

NATURE PUBLISHING GROUP2018

Coherent imaging from bacteria to multicellular organisms

Séverine Coquoz

Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...

EPFL2017

Super-resolution microscopy live cell imaging and image analysis

Tomas Lukes

Novel fundamental research results provided new techniques going beyond the diffraction limit. These recent advances known as super-resolution microscopy have been awarded by the Nobel Prize as they promise new discoveries in biology and live sciences. All ...

Czech Technical University in Prague2017

Compact Lensless Phase Imager

Michaël Unser, Ferréol Arnaud Marie Soulez

Lensless quantitative phase imaging is of high interest for obtaining a large field of view (FOV), typically the size of the camera chip, to observe biological cell material with high contrast. It has the potential to be widely spread due to its inherent s ...

2017

Compact Side and Multi Angle Illumination Lensless Imager and Method of Operating the Same

Christophe Moser, Manon Rostykus

A system for subpixel resolution imaging of an amplitude and quantitative phase image, the system including a waveguide having a top plane, a bottom plane, and two sides, an array of light sources emitting first befit beams from one side of the two sides o ...

2017

Introduction to the Issue on Advanced Signal Processing in Microscopy and Cell Imaging

Michaël Unser

Microscopy imaging, including fluorescence microscopy and electron microscopy, has a prominent role in life science and medical research. During the past two decades, biological imaging has undergone a revolution by way of the development of new microscopy ...

IEEE2016

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Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

Hermite Snakes with Control of Tangents

Michaël Unser, Julien René Pierre Fageot, Virginie Sophie Uhlmann

We introduce a new model of parametric contours defined in a continuous fashion. Our curve model relies on Hermite spline interpolation and can easily generate curves with sharp discontinuities; it also grants direct access to the tangent at each location. ...

IEEE2016

Variational Phase Imaging Using the Transport-of-Intensity Equation

Michaël Unser, Daniel Sage, Emrah Bostan, Masih Nilchian, Emmanuel Augustin Julien Froustey

We introduce a variational phase retrieval algorithm for the imaging of transparent objects. Our formalism is based on the transport-of-intensity equation (TIE), which relates the phase of an optical field to the variation of its intensity along the direct ...

IEEE2016