Publication

Second harmonic phase microscopy of collagen fibers

Related publications (45)

Holographic second harmonic phase microscopy of collagen fibers

Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
2011

Holographic second harmonic generation (SHG) microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

The coherent nature of second harmonic generation (SHG) makes possible retrieval of both its amplitude and its phase, by use of a proper phase-sensitive imaging technique such as digital holographic SHG microscopy. In addition to retrieving both amplitude ...
2011

Holographic Second Harmonic Generation Microscopy

Etienne Shaffer

This dissertation is dedicated to the application of digital holography methods to second harmonic generation microscopy, and to the development of the resulting imaging technique: holographic second harmonic generation microscopy. Second harmonic generati ...
EPFL2011

Novel Concepts for Functional High Resolution Microscopy

Matthias Geissbühler

Functional imaging of biological cells and organs is one of the key techniques that drives new discoveries in life science research and medicine. In this thesis novel concepts for functional imaging at high spatial and temporal resolution are presented. In ...
EPFL2011

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...
Optical Society of America2010

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Christian Depeursinge, Etienne Shaffer

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index ac ...
SPIE2010

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Etienne Shaffer, Corinne Moratal

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...
Optical Society of America2010

Enhanced Second Harmonic Generation in Plasmonic Nanocavities

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We experimentally demonstrate significantly enhanced second harmonic generation using nanoengineered plasmonic nanocavities of core-shell structures (BaTiO3/Au). An enhancement factor of over 500 is measured in the second harmonic scattering efficiency com ...
Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2010

Digital holographic microscopy investigation of second harmonic generated at a glass/air interface

Christian Depeursinge, Nicolas Pavillon, Jonas Kühn, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering complex diffraction wavefields from which it i ...
2009

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