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Marker-free phase nanoscopy

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Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

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Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

OPTICAL SOC AMER2021

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

EPFL2021

High-Resolution Multi-Spectral Imaging With Diffractive Lenses and Learned Reconstruction

Oguzhan Fatih Kar, Can Deniz Bezek

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To overcome these physi ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2021

The adoption of process analytical technologies by the biopharmaceutical industry can reduce the cost of therapeutic drugs and facilitate investigation of new bioprocesses. Control of critical process parameters to retain critical product quality attribute ...

ELSEVIER2021

High-throughput and adaptive super-resolution fluorescence microscopy of organelles

Dora Mahecic

Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...

EPFL2020

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Biomedical optical techniques for intracochlear cellular imaging

Marilisa Romito

Imaging the inner ear microanatomy is of great importance for the assessment of the state of the cells responsible for sound detection. Hearing disorders are mainly due to a malfunction of the cochlea, the bone containing the cells inside the inner ear. Un ...

EPFL2019

Overcoming the classical diffraction limit in optical microscopy is known to be achievable by a variety of far-field and near-field microscopy techniques. More recently, so-called micro-object-based optical super-resolution microscopy techniques have emerg ...

2019

Dielectric-sphere-based microsystem for optical super-resolution imaging

Gergely Huszka

Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...

EPFL2018