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This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...
Sense of touch belongs to one of the five Human Senses. Whereas blind people largely use feeling in everyday life, sighted person mainly focus on hearing and vision. However, haptic interface started being introduced in customers applications, such as smar ...
The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...
We present a new fast active contour for images in 3D microscopy. We introduce a fully parametric design that relies on exponential B-spline bases and allows us to impose a sphere-like topology. The proposed 3D snake can approximate blob-like objects with ...
Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...
Adaptive Optics (AO) improves the efficiency of the optical devices in confocal imaging systems by reducing wavefront aberrations. Aberration is caused by imperfections within the system and reduces the optical signal to noise ratio of the resultant images ...
The subject of this thesis is image restoration, that is, deconvolution and denoising. Our work is motivated by applications in the rapidly expanding field of biological imaging and in particular fluorescence microscopy. Inverse problems in this area typic ...
We propose a new method for performing active contour segmentation based on the statistical prior knowledge of the object to detect. From a binary training set of objects, a statistical map describes the possible shapes of the object by computing the proba ...
Visualization of ice particles in a rectangular cavity and ice slurry flows in a pipe are performed. The form and size of ice particles is investigated by optical microscopy, which permits a statistical evaluation of the geometrical shapes of the ice cryst ...
International Institute of Refrigeration IIR-IIF2000
In this paper we propose a new, interactive technique for the segmentation of elongated structures in images. It is based on the so-called live-wire segmentation paradigm and uses a newly developed steerable filter for computing local ridge strength and or ...
