A Psf-Based Approach To Biplane Calibration In 3D Super-Resolution Microscopy
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In this thesis new imaging approaches for optical microscopy are proposed and studied. They are based on the use of dynamic structured illumination in combination with a demodulation detection concept employing CMOS image detectors. Two particular implemen ...
The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...
We describe two optical systems based on the radial basis function approach to pattern classification. An optical-disk-based system for handwritten character recognition is demonstrated. The optical system computes the Euclidean distance between an unknown ...
This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...
Fourier domain Optical coherence microscopy (FDOCM) offers excellent sensitivity and high axial resolution to image the structure of biological tissue. The depth information is extracted in parallel and allows very high volume acquisition rates. An illumin ...
First experiments of coupling a free electron laser to a scanning near-field optical microscope (SNOM) are presented. To address the question of how important such spectroscopy can be for near-field infrared microscopy, we acquired images of the same regio ...
