Protein-nanoparticle interactions: the effects of surface compositional and structural heterogeneity are scale dependent
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The reversible, oriented immobilization of proteins on solid surfaces is a prerequisite for the investigation of mol. interactions and the controlled formation of supramol. assemblies. This paper describes a generally applicable method using a synthetic ch ...
High signal-to-noise Fourier transform IR (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT3R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein ...
This paper describes the synthesis and supramolecular organization of two novel hybrid diblock copolymers based on poly(ethylene glycol) (PEG) and peptide sequences inspired by the coiled coil protein folding motif. The self-organization of the diblock cop ...
Aspects of conformational transitions, folding, and misfolding of peptides and proteins have recently taken center stage in various domains at the interface of chemistry, biology, and medicine because of their impact on protein misfolding diseases. Due to ...
Rotationally resolved spectra in the 5 nu (1) region of (CH3OH)-C-13 reveal strong vibrational splittings, which by analogy to (CH3OH)-C-12, we assign to a resonance between 5 nu (1) and 4 nu (1)+nu (2). Accordingly, the vibrational dynamics on a subpicose ...
This paper reports the synthesis of rationally designed polypeptides that catalyze the decarboxylation of oxaloacetate via an imine intermediate. The authors det. the secondary structures of the polypeptides by two-dimensional NMR spectroscopy. The authors ...
Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exc ...
To test the effect of NH-C(=S) groups on the stability of beta-peptide secondary structures, the authors have synthesized three beta-thiohexapeptide analogs I (X = S, Y = Z = O; X = Z = S, Y = O; X = Y = Z = S) of H-(beta-HVal-beta-HAla-beta-HLeu)2-OH I (X ...
Cross-correlation between the fluctuations of 13Ca-Ha interactions affects the relaxation behavior of two-spin coherences (zero- and double-quantum coherences) involving the 13Ca nuclei of two successive amino acid residues. The cross-correlation rates are ...
A serotonin 5-HT3 receptor was functionally expressed to high levels and on a large scale in mammalian cells with the Semliki Forest virus system. Conditions were optimized to maximize detergent solubilization of the receptor, while preserving ligand bindi ...