Publications related to Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Marcel Leutenegger

Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...

2010

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Kanat Dukenbayev

A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...

2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Super-resolution fluorescence microscopy based on physical models

François Aguet

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

EPFL2009

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Cédric René Jean Vonesch

The subject of this thesis is image restoration, that is, deconvolution and denoising. Our work is motivated by applications in the rapidly expanding field of biological imaging and in particular fluorescence microscopy. Inverse problems in this area typic ...

EPFL2009

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

Karen Scrivener

The distributions of organic additives in self-leveling flooring compounds (SLCs) were investigated using laser-scanning microscopy for the selective visualization of the additives, which had been previously stained by a fluorescent dye. The distributions ...

2009

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

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Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Comparison of Deconvolution Software: A User Point of View—Part 2

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Three-dimensional nano-localization of single fluorescent emitters

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Super-resolution fluorescence microscopy based on physical models

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

Comparison of Deconvolution Software: A User Point of View—Part 2

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Super-resolution fluorescence microscopy based on physical models

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Three-dimensional nano-localization of single fluorescent emitters

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy