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Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies

Related publications (48)

Middle-down approaches for mass spectrometry-based protein identification and characterization

Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...

EPFL2016

Validation of the Mass-Extraction-Window for Quantitative Methods Using Liquid Chromatography High Resolution Mass Spectrometry

Laure Menin, Anne-Laure Gassner, Bertrand Rochat

A paradigm shift is underway in the field of quantitative liquid chromatography-mass spectrometry (LC-MS) analysis thanks to the arrival of recent high-resolution mass spectrometers (HRMS). The capability of HRMS to perform sensitive and reliable quantific ...

Amer Chemical Soc2016

Extension of least squares spectral resolution algorithm to high-resolution lipidomics data

Adrian Schmid

Lipidomics, which focuses on the global study of molecular lipids in biological systems, has been driven tremendously by technical advances in mass spectrometry (MS) instrumentation, particularly high-resolution MS. This requires powerful computational too ...

Elsevier2016

Middle-down analysis of monoclonal antibodies with electron transfer dissociation Orbitrap FTMS

Yury Tsybin, Luca Fornelli, Daniel Ayoub

The rapid growth of approved biotherapeutics, e.g., monoclonal antibodies or immunoglobulins G (IgGs), demands improved techniques for their quality control. Traditionally, proteolysis-based bottom-up mass spectrometry (MS) has been employed. However, the ...

Amer Chemical Soc2014

Extended Bottom-Up Proteomics with Secreted Aspartic Protease Sap9

Yury Tsybin, Kristina Srzentic, Unige Anna Laskay

We investigate the benefits and experimental feasibility of approaches enabling the shift from short (1.7 kDa on average) peptides in bottom-up proteomics to about twice longer (similar to 3.2 kDa on average) peptides in the so-called extended bottom-up pr ...

Elsevier Science Bv2014

From High- to Super-resolution Mass Spectrometry

Yury Tsybin

High-resolution mass spectrometry (MS) is indispensable for the molecular-level analysis of biological and environmental samples with great intra- and inter-molecular complexity. Here, we summarize developments in Fourier transform mass spectrometry (FTMS) ...

Schweizerische Chemische Gesellschaft2014

Standard addition strip for quantitative electrostatic spray ionization mass spectrometry analysis: Determination of caffeine in drinks

Hubert Girault, Christophe Roussel, Liang Qiao, Elena Tobolkina

Standard addition strips were prepared for the quantitative determination of caffeine in different beverages by electrostatic spray ionisation mass spectrometry (ESTASI-MS). The gist of this approach is to dry spots of caffeine solutions with different con ...

Elsevier2014

From allergen detection to protein-ligand interactions

Natalia Gasilova

Fundamental biological and biochemical studies, proteomics, practical nutrition-related and medical applications, all the research fields, where bioanalytical chemistry is an essential tool, constantly require the improvement of existing techniques of bioa ...

EPFL2014

Structural Analysis of Complex Molecular Systems by High-Resolution and Tandem Mass Spectrometry

Yury Tsybin

In this chapter, we describe the advances in high-resolution and tandem mass spectrometry applied for structural analysis of complex molecular systems. Focus is made on Fourier transform mass spectrometers (FTMS) because of their ability to routinely provi ...

Wiley-VCH Verlag GmbH & Col. KGaA2014

Practical Considerations for Improving the Productivity of Mass Spectrometry-based Proteomics

Yury Tsybin, Anton Kozhinov, Luca Fornelli, Kristina Srzentic, Unige Anna Laskay, Oxana Upir

Mass spectrometry (MS) is currently the most sensitive and selective analytical technique for routine peptide and protein structure analysis. Top-down proteomics is based on tandem mass spectrometry (MS/MS) of intact proteins, where multiply charged precur ...

Schweizerische Chemische Gesellschaft2013

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