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Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae
Related publications (36)
Statistical learning quantifies transposable element-mediated cis-regulation
Didier Trono, Evaristo Jose Planet Letschert, Julien Léonard Duc, Alexandre Coudray, Julien Paul André Pontis, Delphine Yvette L Grun, Cyril David Son-Tuyên Pulver, Shaoline Sheppard
Background: Transposable elements (TEs) have colonized the genomes of most metazoans, and many TE-embedded sequences function as cis-regulatory elements (CREs) for genes involved in a wide range of biological processes from early embryo- genesis to innate ...
2023Natural competence of the pathogen Acinetobacter baumannii: an elusive phenomenon
Natural competence for transformation is an important driver of horizontal DNA exchange between different organisms. This can result in accumulation of dangerous genetic features, such as antibiotic resistance genes, in a single organism. One example of an ...
EPFL2022Chromatin Proteomics to Study Epigenetics - Challenges and Opportunities
Regulation of gene expression is essential for the functioning of all eukaryotic organisms. Understanding gene expression regulation requires determining which proteins interact with regulatory elements in chromatin. MS-based analysis of chromatin has emer ...
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC2021Two nanomachines drive evolution in diverse Vibrio species
Horizontal gene transfer (HGT) has a major impact on bacterial evolution, leading to acquisition or deletion of genes and gene clusters, including those encoding antibiotic resistances and virulence factors. HGT therefore contributes to pathogen emergence, ...
EPFL2019ORGANOHALIDE RESPIRATION: STUDY OF THE MEMBRANE PROTEIN COMPLEX RESPONSIBLE FOR THE REDUCTION OF HALOGENATED COMPOUNDS
Christof Holliger, Julien Maillard, Adrian Schmid, Lorenzo Cimmino
Organohalide respiration (OHR) is a bacterial anaerobic respiratory process in which halogenated compounds are used as terminal electron acceptors. Desulfitobacterium and Dehalobacter, paradigmatic organohalide-respiring bacteria (OHRB), harbour the pceABC ...
2019Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae
Melanie Blokesch, Nicolas Jean Philippe Guex, Christian Iseli, Sandrine Stutzmann, Candice Stoudmann, Noémie Matthey
Natural competence for transformation is a primary mode of horizontal gene transfer. Competent bacteria are able to absorb free DNA from their surroundings and exchange this DNA against pieces of their own genome when sufficiently homologous. However, the ...
2019Mechanisms of DNA Uptake by Naturally Competent Bacteria
Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires a DNA-uptake pilus and the DNA-binding protein ComEA. In the gram-negative bacteria, DNA is first pulled toward the outer m ...
2019Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species
Melanie Blokesch, Candice Stoudmann, Noémie Matthey, Lisa Christina Metzger, Esther Jeanne Collas
Bacteria of the genus Vibrio are common members of aquatic environments where they compete with other prokaryotes and defend themselves against grazing predators. A macromolecular protein complex called the type VI secretion system (T6SS) is used for both ...
2019DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction
Melanie Blokesch, Sandrine Stutzmann, Candice Stoudmann, David William Adams
How bacteria colonize surfaces and how they distinguish the individuals around them are fundamental biological questions. Type IV pili are a widespread and multipurpose class of cell surface polymers. Here we directly visualize the DNA-uptake pilus of Vibr ...
2019The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase
Melanie Blokesch, Sandrine Stutzmann, Candice Stoudmann, David William Adams, Jorge André Matias Pereira
Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these structures to undergo repetitive cycles of extension and retraction underpins their crucial roles in adhesion, motility and natural competence for transfor ...
2019