Publication
Structural and Functional Imaging of Pancreatic Islets using Optical Coherence Microscopy
Related publications (50)
Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets
Jonathan Paz Montoya, Howard Riezman
Recent evidence suggests that circadian clocks ensure temporal orchestration of lipid homeostasis and play a role in pathophysiology of metabolic diseases in humans, including type 2 diabetes (T2D). Nevertheless, circadian regulation of lipid metabolism in ...
PUBLIC LIBRARY SCIENCE2022Multipotent mesenchymal stromal cells derived from porcine exocrine pancreas improve insulin secretion from juvenile porcine islet cell clusters.
Sandrine Gerber, Luca Szabó, Léo Bühler
Neonatal and juvenile porcine islet cell clusters (ICC) present an unlimited source for islet xenotransplantation to treat type 1 diabetes patients. We isolated ICC from pancreata of 14 days old juvenile piglets and characterized their maturation by immuno ...
2021In pancreatic islets from type 2 diabetes patients, the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis
Circadian clocks operative in pancreatic islets participate in the regulation of insulin secretion in humans and, if compromised, in the development of type 2 diabetes (T2D) in rodents. Here we demonstrate that human islet alpha- and beta-cells that bear a ...
NATL ACAD SCIENCES2020Targeting Mitochondrial Calcium Uptake with the Natural Flavonol Kaempferol, to Promote Metabolism/Secretion Coupling in Pancreatic beta-cells
Umberto De Marchi, Guillaume Jacot
Pancreatic beta-cells secrete insulin to lower blood glucose, following a meal. Maintenance of beta-cell function is essential to preventing type 2 diabetes. In pancreatic beta-cells, mitochondrial matrix calcium is an activating signal for insulin secreti ...
2020Role of metabolism and mitochondria during glucose sensing and glucose-induced dysfunction in human pancreatic beta-cells
Glucose sensing and regulation of insulin secretion are the two main functions performed by the pancreatic beta-cell. Together these processes contribute to the tight control of blood glucose in our body. Compromising the ability of the beta-cells to prope ...
EPFL2019Multipotent mesenchymal stromal cells enhance insulin secretion from human islets via N-Cadherin interaction and prolong function of transplanted encapsulated islets in mice
Sandrine Gerber, Jutta Christine Wandrey, Redouan Mahou
Background: Multipotent mesenchymal stromal cells (MSC) enhance viability and function of islets of Langerhans. We aimed to examine the interactions between human MSC and human islets of Langerhans that influence the function of islets. Methods: Human MSC ...
Biomed Central Ltd2017Investigating the Biological Mechanisms underlying the Initiation of Autoimmunity against Beta Cell Antigens in Type 1 Diabetes
Type 1 diabetes (T1D) is an autoimmune disease characterized by circulating autoantibodies, lymphocytic infiltration of pancreatic islets of Langerhans, and cell-specific destruction of beta cells, leading to insulin deficiency and symptomatic hyperglycemi ...
EPFL2017Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation
Olivier Burri, Andreas Wiederkehr, Jeffrey Alan Hubbell, Steinunn Baekkeskov Hanahan, Chiara Cianciaruso, Miriella Carmela Pasquier, Edward Allen Phelps, Gabriele Galliverti
A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and livecell microscopy. Guided by an observation that dispersed isle ...
Springer Nature2017Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow
Theo Lasser, Paul James Marchand, Anne Grapin-Botton, Arno Pino Bouwens, Daniel Pawel Szlag, Corinne Berclaz, Jérôme Extermann, David Nguyen, Julia Sophie Katarina Nilsson
In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...
Optical Soc Amer2016Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers
Theo Lasser, Christophe Pache, Anne Grapin-Botton, Arno Pino Bouwens, Daniel Pawel Szlag, Corinne Berclaz, Antonio Lopez
The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...
2015