Publications related to Study of transposon-mediated cell pool and cell line generation in CHO cells

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

Strategies in non-viral gene delivery: "to overcome serum effect"

Yun Suk Jo

Throughout these last years, many synthetic polymers have been developed for non-viral gene delivery systems in attempt to overcome potential shortcomings of viral vectors. However, it is still difficult to achieve highly efficient transfection under physi ...

2008

Gene delivery from responsive hyaluronic acid hydrogels for directed angiogenesis

Arnaud Felber

Tissue Engineering points to maintain, restore or to improve and create new tissues. This purpose typically involves the selection of a natural or synthetic biomaterial as a scaffold to create the biological substitutes. Furthermore, angiogenesis, the grow ...

2008

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Heinrich Hofmann, Alke Fink, Benedikt Steitz

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocyt ...

2008

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient ge ...

2008

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Florian Maria Wurm, David Hacker, Mathieu Benjamin Stettler, Xiaowei Zhang

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

American Chemical Society2007

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gen ...

Kluwer Academic Publishers, Springer2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, David Hacker, Madiha Derouazi, Martin Jordan, Natalie Muller, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Bo Song, Steve Comby

The homoditopic ligand

H_2L^{C3}

has been designed to form neutral triple-stranded bimetallic helicates of overall composition [

Ln_2(L^{C3})_3

]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration whi ...

2007

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Florian Maria Wurm, Madiha Derouazi, Martin Jordan, Philippe Pierre Girard

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Study of transposon-mediated cell pool and cell line generation in CHO cells

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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Strategies in non-viral gene delivery: "to overcome serum effect"

Gene delivery from responsive hyaluronic acid hydrogels for directed angiogenesis

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Strategies in non-viral gene delivery: "to overcome serum effect"

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Gene delivery from responsive hyaluronic acid hydrogels for directed angiogenesis

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Novel orbital shake bioreactors for transient production of CHO derived IgGs

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Generation of recombinant Chinese hamster ovary cell lines by microinjection