Publications related to Automatic Tracking of Particles in Dynamic Fluorescence Microscopy

Functional and Structural Imaging with Optical Coherence Microscopy

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Jeremy Luterbacher

We report on studies of Thermobifida fusca cellulases Cel5A, Cel6B and Cel9A binding to pretreated wood particles using Confocal Laser Scanning Microscopy (CLSM). Hydro-thermal pretreated wood particles were immobilized on borosilicate substrates before fl ...

2011

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Nanostructured Functional Particles and Surfaces

Arthur Olivier Joseph Ganz

Topographic nanostructuring of surfaces is a promising concept, in particular to improve the bio-integration of titanium orthopedic implants. In this context, a new method for the nanostructuring of anodised titanium surfaces was developed. Ordered topogra ...

EPFL2010

High-resolution microscopy and photolithography devices using focusing micromirrors

René Salathé, Fabrice Merenda, Bastien Rachet

The invention relates to large-field high-resolution microscopy and photolithography setups operating with polychromatic light. It includes the use of a plurality of focusing micromirrors. ...

2010

Super-resolution fluorescence microscopy based on physical models

François Aguet

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

EPFL2009

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

Karen Scrivener

The distributions of organic additives in self-leveling flooring compounds (SLCs) were investigated using laser-scanning microscopy for the selective visualization of the additives, which had been previously stained by a fluorescent dye. The distributions ...

2009

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy

Michaël Unser, Cédric René Jean Vonesch

Wavelet-domain

ℓ _{ 1 }

-regularization is a promising approach to deconvolution. The corresponding variational problem can be solved using a “thresholded Landweber” (TL) algorithm. While this iterative procedure is simple to implement, it is known to ...

IEEE2008

Automatic Tracking of Particles in Dynamic Fluorescence Microscopy

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Functional and Structural Imaging with Optical Coherence Microscopy

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Three-dimensional nano-localization of single fluorescent emitters

Comparison of Deconvolution Software: A User Point of View—Part 2

Nanostructured Functional Particles and Surfaces

High-resolution microscopy and photolithography devices using focusing micromirrors

Super-resolution fluorescence microscopy based on physical models

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy

High-resolution microscopy and photolithography devices using focusing micromirrors

Functional and Structural Imaging with Optical Coherence Microscopy

Super-resolution fluorescence microscopy based on physical models

Quantitative distribution patterns of additives in self-leveling flooring compounds (underlayments) as function of application, formulation and climatic conditions

Nanostructured Functional Particles and Surfaces

Three-dimensional nano-localization of single fluorescent emitters

Comparison of Deconvolution Software: A User Point of View—Part 2