Publications related to Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Heinrich Hofmann, Alke Fink, Benedikt Steitz

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocyt ...

2008

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

Christoph Merten

High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary a ...

2008

Drop-based microfluidic devices for encapsulation of single cells

Christoph Merten

We use microfluidic devices to encapsulate, incubate, and manipulate individual cells in picoliter aqueous drops in a carrier fluid at rates of up to several hundred Hz. We use a modular approach with individual devices for each function, thereby significa ...

2008

Microfluidics-Generated Immobilized Biomolecule Gradients on Hydrogels

Steffen Cosson

Extracellular matrix (ECM)-tethered morphogen gradients play an important role in tissue development and regeneration. There is currently a limited set of model systems that can mimic these gradients in an artificial ECM-like environment. Microfluidics off ...

2008

Microfluidic enzymatic reactors for proteome research

Hubert Girault

The field of proteomics has emerged as a valuable analytical tool for elucidating cellular and biological systems at the molecular level. As there is an ever-growing demand for new highly automated, high-throughput, and sensitive analytical tools, a key ch ...

2008

Optical trapping and micromanipulation of biological objects in microfluidic channels

Marinela Gjoni Boillat

The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. ...

EPFL2007

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Bo Song, Steve Comby

The homoditopic ligand

H_2L^{C3}

has been designed to form neutral triple-stranded bimetallic helicates of overall composition [

Ln_2(L^{C3})_3

]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration whi ...

2007

Bounded Asynchrony: A Biologically Inspired Notion of Concurrency

Maria-Emanuela-Canini Mateescu

We introduce {\em bounded asynchrony}, a notion of `concurrency tailored to the modeling of biological cell-cell interactions. Bounded asynchrony is the result of a scheduler that bounds the number of steps that one process get ahead of other processes; th ...

2007

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

NOVELTY - Determining a receptor-ligand interaction involves repeatedly contacting a cell comprising a receptor, with a ligand, of the receptor in which the ligand has a fluorescence labeling group and binds reversibly to the receptor under conditions in w ...

2006

A versatile tool for conditional gene expression and knockdown

Didier Trono, Patrick Aebischer, Marc-Olivier Sauvain

Drug-inducible systems allowing the control of gene expression in mammalian cells are invaluable tools for genetic research, and could also fulfill essential roles in gene- and cell-based therapy. Currently available systems, however, often have limited in ...

Nature Publishing Group2006

Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

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Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

Drop-based microfluidic devices for encapsulation of single cells

Microfluidics-Generated Immobilized Biomolecule Gradients on Hydrogels

Microfluidic enzymatic reactors for proteome research

Optical trapping and micromanipulation of biological objects in microfluidic channels

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Bounded Asynchrony: A Biologically Inspired Notion of Concurrency

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

A versatile tool for conditional gene expression and knockdown

Drop-based microfluidic devices for encapsulation of single cells

Microfluidic enzymatic reactors for proteome research

Microfluidics-Generated Immobilized Biomolecule Gradients on Hydrogels

Optical trapping and micromanipulation of biological objects in microfluidic channels

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Bounded Asynchrony: A Biologically Inspired Notion of Concurrency

A versatile tool for conditional gene expression and knockdown

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms